Polymorphism of Calpastatin Gene in Crossbreed Dalagh Sheep Using PCR-RFLP
نویسندگان
چکیده
منابع مشابه
PolymorPhism of calPastatin, calPain and myostatin genes in native dalagh sheeP in iran
Calpains play a major role in post mortem tenderization and calpastatin is the endogenous inhibitor of calpain proteases and regulates the rate and extent of post mortem tenderization. Myostatin is an inhibitor of skeletal muscle growth and a mutation in the gene coding region leads to increased muscling. Therefore, they are considered as candidate genes for meat and growth traits. Blood sample...
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The genotypes for calpastatin (CAST) and calpain (CAPN) loci were determined by PCR-RFLP and PCRSSCPmethods. Blood samples were collected from 200 pure-bred Zel sheep from Shirang’s Zel sheep Breeding Station located in south-west of Golestan, Iran. Extraction of genomic DNA was performed based on the modified salting out method. Based on results, two investigated loci were polymorphic an...
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Calpastatin is an endogenous inhibitor of calpain (calcium-dependent cysteine protease). Calpastatin activityis highly related to the rate of protein turnover and rate of meat tenderization. In order to characterize thestructure of calpastatin in Iranian Afshari breed of sheep, intron 6 and partial exon 7 of the L domain wereamplified and sequenced. A fragment of approximately...
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This investigation was conducted to identify polymorphism of the leptin gene in Holstein cows, Mazandarani native cattle and buffaloes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The frequencies of A and B alleles were 0.4 and 0. 6 in Holstein cows, 0.56 and 0.44 in Mazandarani native cattle, 0.61 and 0.35 in buffaloes, respectively. The heterozygote genoty...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2011
ISSN: 1684-5315
DOI: 10.5897/ajb11.265